Intermediate
Dioxy
Part:BBa_K1688022:Design
Designed by: Delyan Georgiev Group: iGEM15_Uppsala (2015-09-16)
Catechol 1,2 Dioxygenase + HlyA export tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 176
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for restriction-free cloning were designed in such a way as to remove the STOP codon of the catechol 1,2 dioxygenase and the scar left from 3A assembly. However, due to a design error, an AG insert between the two parts was introduced, resulting in a stop codon at the beginning of the HlyA sequence.
Source
Restriction free cloning.